DisProt 2024_06 - New thematic dataset of RNA-binding proteins (RBPs)

With this new DisProt thematic dataset, we explore the role of intrinsically disordered proteins (IDPs) binding RNA.

Written on Jun 17, 2024, by Maria Cristina Aspromonte.

New thematic dataset of RNA-binding proteins

Many proteins regulate the expression of genes by binding to specific regions encoded in the genome. Here we introduce a new thematic dataset: RNA-binding proteins (RBPs).

RBPs are a diverse class of proteins that interact with RNA to form ribonucleoprotein complexes, and regulate numerous aspects of gene expression (e.g. pre-mRNA splicing, translation etc) in several organisms. Many experimental data showed that several proteins are intrinsically disordered or contain disordered regions, which play a central role in regulatory processes.

Our dataset includes more than 190 disordered RBPs in Homo sapiens, viruses, bacteria and other organisms. It is enriched with various pieces of evidence to describe disorder function (IDPO), and molecular and biological function (GO). Our dataset includes various examples of fully disordered RBPs as well as those with functionally important disordered regions.

Examples from “RNA-binding proteins” dataset:

  • DP01087 - Protein Tat (Organism: Human immunodeficiency virus type 1) encoded by gene tat. This protein acts by increasing the efficiency of the transcription process in HIV and its activity is influenced by IDRs.
  • DP01474 - Nucleophosmin (Organism: Homo sapiens) encoded by gene NPM1. It’s involved in diverse cellular processes such as ribosome biogenesis, histone assembly, and regulation of tumor suppressors p53/TP53. NPM1 protein contains IDR (aa 120-243) with potential RNA binding activity

Thanks to the effort of expert DisProt biocurators, Maria Victoria Nugnes, Federica Quaglia, and the DisProt Consortium in particular Edoardo Salladini, Julian Bergier, Veronika Ács, Emanuela Leonardi, Alma Carolina Sanchez Rocha. The new dataset features 195 proteins and more than 1800 pieces of evidence obtained from over 156 publications.

Notes: RNA-binding proteins thematic dataset icon was Created with Biorender.com